Investigation of the effects of different genotypes on regeneration capacity in Triticale

Abstract

Triticale (x Triticosecale Wittmack) is a synthetic amphiploid cereal that grows on about 3 million hectares in the world. It is grown mostly for forage or animal feed although some triticale-based foods can be purchased at health food stores or are found in some breakfast cereals. Mature embryos of two triticale cultivars (Ümran Hanım and Melez 2001) were used as the resources explants. Effects of one auxin type (2,4-D) and three various concentrations, (4.0 mg/l; 8mg/l; 12.0mg/l) callus formation and plant regeneration was determined. In terms of results, Ümran Hanım triticale cultivar was observed highest on callus formation and plant regeneration. Callus formation and plant regeneration was affected by 3 different concentration. Callus formation values were detected over 85% in three concentrations. The highest effects were detected in a medium including 4 mg/l 2,4-D solidified with agar. Embryogenic callus formation ratio were detected 32.38%, 12,8%6 and 16,08% respectively.